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4cxg c. Jan 28, 13 · To produce apoRBP, add an equal volume of heptane to the purified holoRBP and mix gently by rotating overnight at 4 °C Centrifuge at 16,000 g for 10 min at 4 °C and carefully transfer the bottom aqueous phase to a new tube (avoiding the precipitate at the interface) Repeat the process three times with a 3 hr incubation for each. Share your videos with friends, family, and the world. Oct 12, 17 · Samples are incubated for 30 min at 37 °C as in step 76 50 µL of fluorochrome reagent are then added into each well as in step 77 (final concentration of 300 µM) The fluorescent readout (in dark) is then assessed every minute over 1 minutes at 37 °C with a fluorescent plate reader (530/590 nm filters).
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